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| Registros recuperados: 149 | |
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| Siqueira,Marcos Vinicius Bohrer Monteiro; Borges,Aline; Valle,Teresa Losada; Veasey,Elizabeth Ann. |
| This study was carried out in order to assess the genetic diversity of 20 cassava (Manihot esculenta Crantz) genotypes with high phenotypic performance using microsatellite markers. Two groups were considered for this study: eleven varieties for industrial uses and nine for household consumption. By using nine microsatellite primers, high polymorphism was identified in all the loci analyzed, with values reaching 100%. On average, 3.4 alleles per locus were found, with 0.371 the value estimated for the observed heterozygosity and 0.555 for gene diversity for the entire set of varieties. The genetic variability found in both varieties, cultivated on a large-scale in the South Center region of Brazil, is wide enough to allow the choice of divergent parental... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Genetic diversity; Germplasm; Microsatellites. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052011000400003 |
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| Pelser, P.B.; Nickrent, D.L.; Barcelona, J.F.. |
| Rafflesia speciosa is a threatened endo-holoparasitic species. It has several populations in the Central Panay Mountain Range (CPMR) of Panay island and a single population on Negros Island. Because R. speciosa is the only Philippine species of the genus that is not endemic to an individual island, it is a suitable species for improving our understanding of the factors underlying the high island endemism of Philippine Rafflesia. For this purpose and to inform the conservation management of R. speciosa, patterns of genetic diversity and differentiation were studied using 15 microsatellite loci and samples from nine populations. None of these populations shows evidence of inbreeding and R. speciosa has similar levels of heterozygosity as generally observed... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Microsatellites; Conservation; Negros; Panay; Philippines; Population genetics. |
| Ano: 2018 |
URL: http://www.repository.naturalis.nl/record/655789 |
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| Lallias, Delphine. |
| Cette étude vise à approfondir la connaissance de la biologie de la reproduction de l'huître plate Ostrea edulis afin de mieux comprendre comment les caractéristiques de la biologie de reproduction de l'espèce peuvent rendre compte des phénomènes de déficits en hétérozygote et de relation positive entre hétérozygotie et croissance. En particulier, elle vise à analyser le succès reproducteur en milieu naturel et dans les populations maintenues en écloserie. Cette étude repose sur l'utilisation de marqueurs microsatellites qui, du fait de leur fort polymorphisme, représentent des outils puissants d'assignation de parenté. Une première partie de l'étude a consisté à analyser les pontes issues de femelles incubantes prélevées dans le milieu naturel. Le nombre... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Microsatellites; Biologie de la reproduction; Variance du succès reproducteur. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00370/48167/48281.pdf |
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| Griot, Ronan; Allal, Francois; Brard‐fudulea, S; Morvezen, R; Haffray, P; Phocas, F; Vandeputte, Marc. |
| In the context of parentage assignment using genomic markers, key issues are genotyping errors and an absence of parent genotypes because of sampling, traceability or genotyping problems. Most likelihood‐based parentage assignment software programs require a priori estimates of genotyping errors and the proportion of missing parents to set up meaningful assignment decision rules. We present here the R package APIS, which can assign offspring to their parents without any prior information other than the offspring and parental genotypes, and a user‐defined, acceptable error rate among assigned offspring. Assignment decision rules use the distributions of average Mendelian transmission probabilities, which enable estimates of the proportion of offspring with... |
| Tipo: Text |
Palavras-chave: Microsatellites; Missing parents; Parentage assignment; Pedigree; SNP. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00587/69866/67767.pdf |
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| Faria,Patrícia J.; Baus,Erika; Morgante,João S.; Bruford,Michael W.. |
| Little information is available about the population structure of communally nesting terns (Sternidae) and skimmers (Rynchopidae) throughout the world. In order to fill this gap, a survey of molecular markers was carried out for six species of terns (Anous stolidus, Sterna hirundinacea, S. fuscata, S. superciliaris, Thalasseus maximus and Phaetusa simplex) and one species of skimmer (Rynchops niger). First, we describe the results of the construction of genomic DNA libraries and document problems encountered during this procedure. Secondly, we tested the cross-amplification of 18 microsatellite loci previously described for related species (the number of polymorphic loci ranged from three to seven). Thirdly, we tested the usefulness of mtDNA (control... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; Microsatellites; Mitochondrial DNA; Terns. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400029 |
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| Caparroz,Renato; Mantellatto,Aline M.B.; Bertioli,David J.; Figueiredo,Marina G.; Duarte,José Maurício B.. |
| The complete mitochondrial genome of the brown brocket deer Mazama gouazoubira and a set of polymorphic microsatellite markers were identified by 454-pyrosequencing. De novo genome assembly recovered 98% of the mitochondrial genome with a mean coverage of 9-fold. The mitogenome consisted of 16,356 base pairs that included 13 protein-coding genes, two ribosomal subunit genes, 22 transfer RNAs and the control region, as found in other deer. The genetic divergence between the mitogenome described here and a previously published report was ∼0.5%, with the control region and ND5 gene showing the highest intraspecific variation. Seven polymorphic loci were characterized using 15 unrelated individuals; there was moderate genetic variation across most loci (mean... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Microsatellites; Mitogenome; Population genetics; 454-pyrosequencing. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300338 |
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| Souza,Silvia Graciele Hülse de; Carpentieri-Pípolo,Valéria; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Ruas,Paulo Maurício; Gerage,Antônio Carlos. |
| The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinates analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Corn; Distance genetic; Microsatellites; Molecular markers; Polymorphism; RAPD. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100022 |
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| Nikolic, Natacha; Chevalet, Claude. |
| Understanding and predicting population abundance is a major challenge confronting scientists. Several genetic models have been developed using microsatellite markers to estimate the present and ancestral effective population sizes. However, to get an overview on the evolution of population requires that past fluctuation of population size be traceable. To address the question, we developed a new model estimating the past changes of effective population size from microsatellite by resolving coalescence theory and using approximate likelihoods in a Monte Carlo Markov Chain approach. The efficiency of the model and its sensitivity to gene flow and to assumptions on the mutational process were checked using simulated data and analysis. The model was found... |
| Tipo: Text |
Palavras-chave: Atlantic salmon; Effective population size; Microsatellites; Past demography. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00194/30565/28986.pdf |
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| Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry. |
| Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067 |
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| Registros recuperados: 149 | |
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